Journal: Malaria Journal

Article Title: Improvement of malaria diagnostic system based on acridine orange staining

doi: 10.1186/s12936-018-2214-8

Figure Lengend Snippet: a Schema for the revised AO staining and slide examination. The AO droplet (indicated by the red open circle) on a cover slip is placed at the tip of the thin film. A typical scanning route to find parasites is indicated by the meandering line. b Picture of a blue LED lamp placed under the stage of a microscope. The LED lamp has a built-in shortpass filter (#) under its condenser lens and a longpass filter (*) is inserted in the microscope body

Article Snippet: Starting with a commercially available flashlight comprising condenser lens and a 3-AAA battery compartment, the original lamp was replaced by a 3 W blue LED of 465–475 nm and an interference shortpass filter (VIS 490 nm, Asahi Spectra, Japan) was inserted under the lens to block weak long-wavelength light component with > 520 nm.

Techniques: Staining, Microscopy